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27 Uppsatser om Synthesized peptides - Sida 1 av 2
Bioactive peptides in long-time ripened open texture semi-hard cheese
Bovine milk is an excellent source of bioactive peptides. The bioactive peptides are in an inactive state in the protein and are released during enzymatic activity, e.g. during cheese ripening. Specific properties of bioactive peptides from ripened cheese has been reported, e.g. antihypertensive, antioxidative, antimicrobial, immunomodulatory and mineral binding.
Breed differences in natriuretic peptides in healthy dogs
Measurement of plasma concentrations of natriuretic peptides have been suggested to be of diagnostic and prognostic value in canine cardiac disease. In several previous canine studies, however, a considerable overlap between investigated groups has been identified. A reliable natriuretic peptide test for use in clinical practice requires establishment of reference values for natriuretic peptides in healthy dogs and cut-off values for subclinical disease as well as for clinical signs of congestive heart failure. Numerous physiological, pathological and pharmacological factors, aside heart function, may influence concentration of natriuretic peptides. A potential effect of dog breed on natriuretic peptide concentration has been suggested but has, to our knowledge, not previously been specifically studied.
Quantification of Tripeptidyl-peptidase II : Optimisation and evaluation of 3 assays
Abstract Tripeptidyl-peptidase II (TPPII), is present in most eukaryotic cells. It cuts tripeptides from the N-terminus of peptides and is especially important for degrading peptides longer than 15 amino acids. TPPII also tailors long peptides into suitable substrates for the enzymes which transport and produce the peptides that MHC I present. Increased levels of TPPII have also been found in certain cancer cells, thus it is of interest to determine if TPPII could be used as a tumour marker.The aim of this study was to optimise and evaluate 3 different methods for quantifying TPPII. Western blot, enzyme-linked immunosorbent assay (ELISA) and fluorophore-linked immunosorbent assay (FLISA) protocols were optimised regarding incubation times and antibody dilutions.
Flavour improvement of water solutions comprising bitter amino acids
The aim of this project was to improve taste of a liquid food product, comprising extremelybitter and unpalatable amino acids and one unrevealed ingredient. The amino acids were Lleucine,L-isoleucine, L-valine, L-threonine and L-lysine monohydrochloride. Experimentaldesign was set up as a screening of water solutions comprising these ingredients (called BCAAsolutions) and added ingredients with potential to improve flavour. BCAA solution withingredients that were potential bitterness suppressors were evaluated in flavour, bitternessand/or palatability. Solutions of separate amino acids were also studied.
The role of FGFs in the elicitation of different cellular responses
Fibroblast growth factors are signaling peptides that controls important cellular processes such as proliferation, differentiation, migration, adhesion and survival. Through binding to different types of receptors on the cell surface, these peptides can have different effect on the target cell. Which effect that is achieved depends on many factors. Thus, each of the 24 known FGFs different biological responses. The FGF receptors (FGFR 1-4) initiates different intracellular pathways which in turn leads to different biological responses.
Inverse Discrete Cosine Transform by Bit Parallel Implementation and Power Comparision
The goal of this project was to implement and compare Invere Discrete Cosine Transform using three methods i.e. by bit parallel, digit serial and bit serial. This application describes a one dimensional Discrete Cosine Transform by bit prallel method and has been implemented by 0.35 ìm technology. When implementing a design, there are several considerations like word length etc. were taken into account.
Att leva med PCOS
Aim: To describe the lived experience of women with polycystic ovary syndrome (PCOS). Method: Eight scientific articles of appropriate quality were found, analyzed, condensed and synthesized. Different experiences by women with PCOS were found and ordered into themes. The findings were then anchored in associated literature and discussed. Results: Four different themes were found: Feeling different; Disturbing symptoms; Searching for answers; Care treatment.
Kvinnors upplevelser av anorexia nervosa : En systematisk litteraturstudie
Background: Anorexia Nervosa (AN) is a complex condition with high mortality. AN has increased within the last fifty years and it?s associated with considerable suffering.Aim: To describe women?s experiences of AN.Method: This study was made as a systematic literature review. Articles were searched in Chinal, PubMed and PsycINFO. Eleven articles were used.
Expression and Purification of Murine Tripeptidyl Peptidase II
Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E.
Detektion av mRNA från en cytokrom c gen belägen i genklustret för kloratmetabolism i Ideonella dechloratans
Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.
Mjölk, gluten och ADHD : En litteraturundersökning om mjölk och glutens påverkan hos barn med ADHD
Attention Deficit Hyperactivity Disorder (ADHD) is becoming a more common diagnosis of younger children. In recent years the perception that some ingredients in our food may have a negative effect regarding the symptoms in children with ADHD has grown stronger. Children with ADHD often suffer from irritated bowel syndromes which affect their ability to digest food. This may result in malnutrition as well as a release of substances that are harmful.The purpose of this report is to compile and illustrate the knowledge of how special food, particular milk protein and gluten, may affect the symptoms of children with ADHD. Also, the report aims to evaluate if there should be changes made in Kindergarten to increase the well-being of these children.
Analysis of Raspberry ringspot virus in raspberry by amplification of RNA-2
So far, only limited research has been carried out on viruses infecting raspberry, even though the diseases that these viruses cause result in economical losses for raspberry growers. Raspberry ringspot virus (RpRSV) belongs to the family Comoviridae and the genus Nepovirus. Nepoviruses are characterized by their icosahedral virus particles and bipartite genome of single-stranded (+) RNA (RNA-1 and RNA-2). These viruses are transmitted mainly by nematodes of the genus Longidorus, which feed on the roots of plants. RpRSV causes disease in raspberry, grapevine, strawberry and many other plants.
Analys av parathormon och joniserat kalcium i blod hos hund :
The calcium homeostasis in the body is normally strict regulated to be kept within very narrow limits. This regulation is due to the action of parathyroid hormone (PTH) which is synthesized in the chief cells of the parathyroid glands. In different diseases, the calcium homeostasis is disturbed and this could either be due to a dysfunction of the parathyroid gland itself, or it could have a completely different explanation. To investigate disturbed calciumhomeostasis, ionized calcium and PTH can be analyzed from a blood sample. There are a lot of different assays to measure PTH in humans.
Urea som kvävekälla till växande ungnöt
The purpose of this study was to examine whether urea could be an option as a nitrogen source for young cattle, the study was limited to risks related to feeding, growth and feed efficiency. Microbes in ruminants have the ability to convert nitrogen to protein, this has been highlighted by beef producers as they are in search of cheap protein feed resources. Urea can be a worthy source of nitrogen for growing young animals, because of its high nitrogen content and the low price. Studies have shown that the replacement of a portion of the total protein content by urea in diets with low content of crude protein, like corn silage, may improve animal growth and feed efficiency. Replacement of all the true protein with urea can result in reduced feed intake and microbial growth.
Döden som profession : Sjuksköterskans upplevelser av att vårda patienter i livets slut ? en litteraturöversikt
Background The aim of palliative care is to improve the quality of life for both the patient and her next of kin during the patient?s end of life. Palliative care rests on the four cornerstones managing symptoms, communication and relationship, teamwork and relative support. The nurses? task is to assess the patients? physical, mental and spiritual needs according to these four cornerstones.Aim To assess nurses? experiences of treating patients at the end of life.Method Eleven academic journals that respond to the aim has been analyzed and synthesized in a literature review in order to summarize current research on the subject.